Josh Tycko


Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy


Contact

Josh Tycko


Contact description



Department of Neurobiology


Harvard Medical School




Josh Tycko


Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



Department of Neurobiology


Harvard Medical School



Publications



Selected Research Articles


Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.


Matthew Durrant*, Alison Fanton*, Josh Tycko*, Michaela Hinks, Sita Chandrasekaran, Nicholas T Perry, Julia M. Schaepe, Peter P. Du, Peter Lotfy, Michael Bassik, Lacramioara Bintu, Ami Bhatt, Patrick Hsu


Nature Biotechnology, 2022


High-Throughput Discovery and Characterization of Human Transcriptional Effectors


Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, A. Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn K. Spees, Peter H. Suzuki, G. Marinov, A. Kundaje, M. Bassik#, Lacramioara Bintu#


Cell, 2020


Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements


Josh Tycko*, M. Wainberg*, G. Marinov*, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa N Truong, Alexandro E. Trevino, Kaitlyn K. Spees, David Yao, Irene M. Kaplow, P. Greenside, D. Morgens, D. Phanstiel, M. Snyder, Lacramioara Bintu, W. Greenleaf, A. Kundaje, M. Bassik


Nature Communications, 2019


Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells


Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, J. Gootenberg, O. Abudayyeh, V. Myer, Christopher J. Wilson, P. Hsu


Nature Communications, 2018


Reviews


Gene drives for schistosomiasis transmission control


T. Maier, Nicolas J. Wheeler, E. Namigai, Josh Tycko, R. Grewelle, Y. Woldeamanuel, Katharina Klohe, J. Perez-Saez, S. Sokolow, G. D. De Leo, T. Yoshino, M. Zamanian, J. Reinhard-Rupp


PLoS Neglected Tropical Diseases, 2019


Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.


Gaelen T. Hess*, Josh Tycko*, David Yao*, M. Bassik


Molecular Cell, 2017


Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology


Josh Tycko*, Mike V. Van*, Michael B. Elowitz, Lacramioara Bintu


Current Opinion in Biomedical Engineering, vol. 4, 2017 Oct 30, pp. 174-193


Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.


Josh Tycko, Vic Myer, Patrick Hsu


Molecules and Cells, 2016


Lessons Learned from the Clinical Development and Market Authorization of Glybera


Laura M. Bryant*, Devin M. Christopher*, April R. Giles*, Christian Hinderer*, Jesse L. Rodriguez*, Jenessa B. Smith*, Elizabeth A. Traxler*, Josh Tycko*, Adam P. Wojno*, James M. Wilson


Human gene therapy. Clinical development, vol. 24(2), 2013 Apr 12, pp. 55-64


Additional Research Articles


Large-scale mapping and systematic mutagenesis of human transcriptional effector domains


Nicole DelRosso, Josh Tycko, Peter H. Suzuki, C. Andrews, Aradhana, Aditya Mukund, Ivan Liongson, Connor Ludwig, Kaitlyn K. Spees, P. Fordyce, M. Bassik, Lacramioara Bintu


bioRxiv, 2022


Modeling the efficacy of CRISPR gene drive for snail immunity on schistosomiasis control


R. Grewelle, J. Perez-Saez, Josh Tycko, E. Namigai, Chloe Rickards, G. D. De Leo


PLoS Neglected Tropical Diseases, 2022


Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)


G. Marinov, Samuel H. Kim, S. Bagdatli, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, M. Bassik, Chuan He, A. Kundaje, W. Greenleaf


bioRxiv, 2021


Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.


Josh Tycko, V. Adam, M. Crosariol, J. Ohlstein, Julio C. Sanmiguel, A. Tretiakova, Soumitra Roy, S. Worgall, James M. Wilson, M. Limberis


Human gene therapy, 2021


A genome-wide genetic screen uncovers novel determinants of human pigmentation


V. Bajpai, T. Swigut, Jaaved Mohammed, Josh Tycko, S. Naqvi, M. Arreola, Tayne C. Kim, Neha Arora, J. Pritchard, M. Bassik, J. Wysocka


bioRxiv, 2021


Identification of Guide-Intrinsic Determinants of Cas9 Specificity


Nicholas C. Huston, Josh Tycko, Eric L Tillotson, Christopher J. Wilson, V. Myer, H. Jayaram, Barrett E Steinberg


The CRISPR Journal, 2019


Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements


Maxwell R. Mumbach, Ansuman T. Satpathy, E. Boyle, Chao Dai, Benjamin G. Gowen, S. Cho, M. Nguyen, Adam J. Rubin, Jeffrey M Granja, Katelynn R. Kazane, Yuning Wei, Trieu Nguyen, P. Greenside, M. Corces, Josh Tycko, Dimitre R. Simeonov, Nabeela Suliman, Rui Li, Jin Xu, Ryan A. Flynn, A. Kundaje, P. Khavari, A. Marson, J. Corn, T. Quertermous, W. Greenleaf, Howard Y. Chang


Nature Genetics, 2017


Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.


Michael S. Magaraci, Jessica G Bermudez, Deeksha Yogish, D. H. Pak, Viktor Mollov, Josh Tycko, D. Issadore, Sevile G. Mannickarottu, B. Chow


ACS synthetic biology, 2016

Share