Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.

Matthew Durrant*, Alison Fanton*, Josh Tycko*, Michaela Hinks, Sita Chandrasekaran, Nicholas T Perry, Julia M. Schaepe, Peter P. Du, Peter Lotfy, Michael Bassik, Lacramioara Bintu, Ami Bhatt, Patrick Hsu

Nature Biotechnology, 2022

High-Throughput Discovery and Characterization of Human Transcriptional Effectors

Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, A. Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn K. Spees, Peter H. Suzuki, G. Marinov, A. Kundaje, M. Bassik#, Lacramioara Bintu#

Cell, 2020

Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Josh Tycko*, M. Wainberg*, G. Marinov*, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa N Truong, Alexandro E. Trevino, Kaitlyn K. Spees, David Yao, Irene M. Kaplow, P. Greenside, D. Morgens, D. Phanstiel, M. Snyder, Lacramioara Bintu, W. Greenleaf, A. Kundaje, M. Bassik

Nature Communications, 2019

Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, J. Gootenberg, O. Abudayyeh, V. Myer, Christopher J. Wilson, P. Hsu

Nature Communications, 2018

Gene drives for schistosomiasis transmission control

T. Maier, Nicolas J. Wheeler, E. Namigai, Josh Tycko, R. Grewelle, Y. Woldeamanuel, Katharina Klohe, J. Perez-Saez, S. Sokolow, G. D. De Leo, T. Yoshino, M. Zamanian, J. Reinhard-Rupp

PLoS Neglected Tropical Diseases, 2019

Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.

Gaelen T. Hess*, Josh Tycko*, David Yao*, M. Bassik

Molecular Cell, 2017

Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology

Josh Tycko*, Mike V. Van*, Michael B. Elowitz, Lacramioara Bintu

Current Opinion in Biomedical Engineering, vol. 4, 2017 Oct 30, pp. 174-193

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.

Josh Tycko, Vic Myer, Patrick Hsu

Molecules and Cells, 2016

Lessons Learned from the Clinical Development and Market Authorization of Glybera

Laura M. Bryant*, Devin M. Christopher*, April R. Giles*, Christian Hinderer*, Jesse L. Rodriguez*, Jenessa B. Smith*, Elizabeth A. Traxler*, Josh Tycko*, Adam P. Wojno*, James M. Wilson

Human gene therapy. Clinical development, vol. 24(2), 2013 Apr 12, pp. 55-64

Large-scale mapping and systematic mutagenesis of human transcriptional effector domains

Nicole DelRosso, Josh Tycko, Peter H. Suzuki, C. Andrews, Aradhana, Aditya Mukund, Ivan Liongson, Connor Ludwig, Kaitlyn K. Spees, P. Fordyce, M. Bassik, Lacramioara Bintu

bioRxiv, 2022

Modeling the efficacy of CRISPR gene drive for snail immunity on schistosomiasis control

R. Grewelle, J. Perez-Saez, Josh Tycko, E. Namigai, Chloe Rickards, G. D. De Leo

PLoS Neglected Tropical Diseases, 2022

Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)

G. Marinov, Samuel H. Kim, S. Bagdatli, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, M. Bassik, Chuan He, A. Kundaje, W. Greenleaf

bioRxiv, 2021

Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.

Josh Tycko, V. Adam, M. Crosariol, J. Ohlstein, Julio C. Sanmiguel, A. Tretiakova, Soumitra Roy, S. Worgall, James M. Wilson, M. Limberis

Human gene therapy, 2021

A genome-wide genetic screen uncovers novel determinants of human pigmentation

V. Bajpai, T. Swigut, Jaaved Mohammed, Josh Tycko, S. Naqvi, M. Arreola, Tayne C. Kim, Neha Arora, J. Pritchard, M. Bassik, J. Wysocka

bioRxiv, 2021

Identification of Guide-Intrinsic Determinants of Cas9 Specificity

Nicholas C. Huston, Josh Tycko, Eric L Tillotson, Christopher J. Wilson, V. Myer, H. Jayaram, Barrett E Steinberg

The CRISPR Journal, 2019

Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements

Maxwell R. Mumbach, Ansuman T. Satpathy, E. Boyle, Chao Dai, Benjamin G. Gowen, S. Cho, M. Nguyen, Adam J. Rubin, Jeffrey M Granja, Katelynn R. Kazane, Yuning Wei, Trieu Nguyen, P. Greenside, M. Corces, Josh Tycko, Dimitre R. Simeonov, Nabeela Suliman, Rui Li, Jin Xu, Ryan A. Flynn, A. Kundaje, P. Khavari, A. Marson, J. Corn, T. Quertermous, W. Greenleaf, Howard Y. Chang

Nature Genetics, 2017

Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.

Michael S. Magaraci, Jessica G Bermudez, Deeksha Yogish, D. H. Pak, Viktor Mollov, Josh Tycko, D. Issadore, Sevile G. Mannickarottu, B. Chow

ACS synthetic biology, 2016