Josh Tycko

Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



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Josh Tycko

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Department of Neurobiology

Harvard Medical School




Josh Tycko

Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



Department of Neurobiology

Harvard Medical School



Publications



Selected Research Articles


Development of compact transcriptional effectors using high-throughput measurements in diverse contexts


Josh Tycko, Mike V. Van, Aradhana, Nicole DelRosso, David Yao, Xiaoshu Xu, Connor Ludwig, Kaitlyn K. Spees, Kathy Liu, Gaelen T. Hess, Mingxin Gu, Aditya Mukund, Peter H. Suzuki, Roarke A. Kamber, Lei S. Qi, Lacramioara Bintu, M. Bassik

bioRxiv, 2023


Large-scale mapping and mutagenesis of human transcriptional effector domains


Nicole DelRosso, Josh Tycko, Peter H. Suzuki, Cecelia Andrews, Aradhana, Aditya Mukund, Ivan Liongson, Connor Ludwig, Kaitlyn K. Spees, P. Fordyce, M. Bassik, Lacramioara Bintu

Nature, 2023


Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.


Matthew Durrant*, Alison Fanton*, Josh Tycko*, Michaela Hinks, Sita Chandrasekaran, Nicholas T Perry, Julia M. Schaepe, Peter P. Du, Peter Lotfy, Michael Bassik, Lacramioara Bintu, Ami Bhatt, Patrick Hsu

Nature Biotechnology, 2022


High-Throughput Discovery and Characterization of Human Transcriptional Effectors


Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, A. Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn K. Spees, Peter H. Suzuki, G. Marinov, A. Kundaje, M. Bassik#, Lacramioara Bintu#

Cell, 2020


Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements


Josh Tycko*, M. Wainberg*, G. Marinov*, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa N Truong, Alexandro E. Trevino, Kaitlyn K. Spees, David Yao, Irene M. Kaplow, P. Greenside, D. Morgens, D. Phanstiel, M. Snyder, Lacramioara Bintu, W. Greenleaf, A. Kundaje, M. Bassik

Nature Communications, 2019


Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells


Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, J. Gootenberg, O. Abudayyeh, V. Myer, Christopher J. Wilson, P. Hsu

Nature Communications, 2018


Reviews


The sound of silence: Transgene silencing in mammalian cell engineering.


A Cabera*, HI Edelstein*, F Glykofrydis*, KS Love*, S Palacios*, J Tycko*, M Zhang*, S Lensch, CE Shields, M Livingston, R Weiss, H Zhao, K Haynes, L Morsut, YY Chen, AS Khalil, W Wong, J Collins, S Rosser, K Polizzi, M Elowitz, M Fussenegger, IB Hilton, JN Leonard, L Bintu, KE Galloway, TL Deans

Cell Systems, 2022


Gene drives for schistosomiasis transmission control


T. Maier, Nicolas J. Wheeler, E. Namigai, Josh Tycko, R. Grewelle, Y. Woldeamanuel, Katharina Klohe, J. Perez-Saez, S. Sokolow, G. D. De Leo, T. Yoshino, M. Zamanian, J. Reinhard-Rupp

PLoS Neglected Tropical Diseases, 2019


Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.


Gaelen T. Hess*, Josh Tycko*, David Yao*, M. Bassik

Molecular Cell, 2017


Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology


Josh Tycko*, Mike V. Van*, Michael B. Elowitz, Lacramioara Bintu

Current Opinion in Biomedical Engineering, vol. 4, 2017 Oct 30, pp. 174-193


Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.


Josh Tycko, Vic Myer, Patrick Hsu

Molecules and Cells, 2016


Lessons Learned from the Clinical Development and Market Authorization of Glybera


Laura M. Bryant*, Devin M. Christopher*, April R. Giles*, Christian Hinderer*, Jesse L. Rodriguez*, Jenessa B. Smith*, Elizabeth A. Traxler*, Josh Tycko*, Adam P. Wojno*, James M. Wilson

Human gene therapy. Clinical development, vol. 24(2), 2013 Apr 12, pp. 55-64


Additional Research Articles


CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping


G. Marinov, Samuel H. Kim, S. Bagdatli, Soon Il Higashino, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, M. Bassik, Chuan He, A. Kundaje, W.J. Greenleaf

Genome Biology, 2023


High-throughput functional characterization of combinations of transcriptional activators and repressors


Aditya Mukund, Josh Tycko, Sage J. Allen, S. A. Robinson, Cecelia Andrews, Connor Ludwig, Kaitlyn K. Spees, M. Bassik, Lacramioara Bintu

bioRxiv, 2022


High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells


Connor H. Ludwig, A. Thurm, D. Morgens, Kevin J. Yang, Josh Tycko, M. Bassik, B. Glaunsinger, Lacramioara Bintu

bioRxiv, 2022


Modeling the efficacy of CRISPR gene drive for snail immunity on schistosomiasis control


R. Grewelle, J. Perez-Saez, Josh Tycko, E. Namigai, Chloe Rickards, G. D. De Leo

PLoS Neglected Tropical Diseases, 2022


Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.


Josh Tycko, V. Adam, M. Crosariol, J. Ohlstein, Julio C. Sanmiguel, A. Tretiakova, Soumitra Roy, S. Worgall, James M. Wilson, M. Limberis

Human gene therapy, 2021


A genome-wide genetic screen uncovers novel determinants of human pigmentation


V. Bajpai, T. Swigut, Jaaved Mohammed, Josh Tycko, S. Naqvi, M. Arreola, Tayne C. Kim, Neha Arora, J. Pritchard, M. Bassik, J. Wysocka

bioRxiv, 2021


Identification of Guide-Intrinsic Determinants of Cas9 Specificity


Nicholas C. Huston, Josh Tycko, Eric L Tillotson, Christopher J. Wilson, V. Myer, H. Jayaram, Barrett E Steinberg

The CRISPR Journal, 2019


Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements


Maxwell R. Mumbach, Ansuman T. Satpathy, E. Boyle, Chao Dai, Benjamin G. Gowen, S. Cho, M. Nguyen, Adam J. Rubin, Jeffrey M Granja, Katelynn R. Kazane, Yuning Wei, Trieu Nguyen, P. Greenside, M. Corces, Josh Tycko, Dimitre R. Simeonov, Nabeela Suliman, Rui Li, Jin Xu, Ryan A. Flynn, A. Kundaje, P. Khavari, A. Marson, J. Corn, T. Quertermous, W. Greenleaf, Howard Y. Chang

Nature Genetics, 2017


Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.


Michael S. Magaraci, Jessica G Bermudez, Deeksha Yogish, D. H. Pak, Viktor Mollov, Josh Tycko, D. Issadore, Sevile G. Mannickarottu, B. Chow

ACS synthetic biology, 2016

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