Josh Tycko


Systematic exploration of molecular functions in human cells to understand gene regulation and enable gene therapy


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Josh Tycko


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Curriculum vitae


Department of Genetics


Stanford University




Josh Tycko


Systematic exploration of molecular functions in human cells to understand gene regulation and enable gene therapy


Department of Genetics


Stanford University



Publications


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Research Articles


Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.


Josh Tycko, Virginie S Adam, Marco Crosariol, Jason Ohlstein, Julio Sanmiguel, Anna P Tretiakova, Soumitra Roy, Stefan Worgall, James M Wilson, Maria P Limberis


Human Gene Therapy, 2021 Jul 19


Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)


Marinov Gk, Kim Sh, Bagdatli St, Trevino Ae, Tycko J, Wu T, Bintu L, Bassik Mc, He C, He C, Kundaje A, Greenleaf Wj


bioRxiv, 2021 Mar 16


High-Throughput Discovery and Characterization of Human Transcriptional Effectors.


Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, Abhimanyu Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn Spees, Peter Suzuki, Georgi K. Marinov, Anshul Kundaje, Michael C. Bassik, Lacramioara Bintu


Cell, vol. 183(7), 2020 Nov 22


Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements


Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik


Nature Communications, vol. 10(1), 2019 Aug 5


Identification of Guide-Intrinsic Determinants of Cas9 Specificity.


Nicholas C. Huston, Josh Tycko, Eric L. Tillotson, Christopher J. Wilson, Vic E. Myer, Hariharan Jayaram, Barrett E. Steinberg


The CRISPR Journal, vol. 2(3), 2019 Apr 31, pp. 172-185


Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells.


Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu


Nature Communications, vol. 9(1), 2018 Jun 26


Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements


Maxwell R Mumbach, Ansuman T Satpathy, Evan A Boyle, Chao Dai, Benjamin G Gowen, Seung Woo Cho, Michelle L Nguyen, Adam J Rubin, Jeffrey M Granja, Katelynn R Kazane, Yuning Wei, Trieu Nguyen, Peyton G Greenside, M Ryan Corces, Josh Tycko, Dimitre R Simeonov, Nabeela Suliman, Rui Li, Jin Xu, Ryan A Flynn, Anshul Kundaje, Paul A Khavari, Alexander Marson, Alexander Marson, Jacob E Corn, Thomas Quertermous, William J Greenleaf, Howard Y Chang


Nature Genetics, vol. 49(11), 2017 Aug 24, pp. 1602-1612



Reviews


Gene drives for schistosomiasis transmission control


Theresa Maier, Nicolas James Wheeler, Nicolas James Wheeler, Erica K. O. Namigai, Josh Tycko, Richard Ernest Grewelle, Yimtubezinash Woldeamanuel, Katharina Klohe, Javier Perez-Saez, Susanne H. Sokolow, Susanne H. Sokolow, Giulio A. De Leo, Timothy P. Yoshino, Mostafa Zamanian, Jutta Reinhard-Rupp


PLOS Neglected Tropical Diseases, vol. 13(12), 2019 Nov 18


Advancing towards a global mammalian gene regulation model through single-cell analysis and synthetic biology


Josh Tycko, Mike V. Van, Michael B. Elowitz, Lacramioara Bintu


Current Opinion in Biomedical Engineering, 2017 Oct 30, pp. 174-193


Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes


Gaelen T. Hess, Josh Tycko, David Yao, Michael C. Bassik


Molecular Cell, vol. 68(1), 2017 Sep 4, pp. 26-43


Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity


Josh Tycko, Vic E. Myer, Patrick D. Hsu


Molecular Cell, vol. 63(3), 2016 Jul 3, pp. 355-370


Lessons Learned from the Clinical Development and Market Authorization of Glybera


Laura M. Bryant, Devin M. Christopher, April R. Giles, Christian Hinderer, Jesse L. Rodriguez, Jenessa B. Smith, Elizabeth A. Traxler, Josh Tycko, Adam P. Wojno, James M. Wilson


Human gene therapy. Clinical development, vol. 24(2), 2013 Apr 12, pp. 55-64



Other


317. Screening S. Aureus CRISPR-Cas9 Paired-Guide RNAs for Efficient Targeted Deletion in Duchenne Muscular Dystrophy


Josh Tycko, Nick Huston, Jacqueline N. Robinson-Hamm, Chris Wilson, Charles A. Gersbach, Patrick D. Hsu, David Bumcrot


Molecular Therapy, 2016 Mar 30