Josh Tycko


Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy


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Josh Tycko


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Department of Neurobiology


Harvard Medical School




Josh Tycko


Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



Department of Neurobiology


Harvard Medical School



Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.


Journal article


Josh Tycko, V. Adam, M. Crosariol, J. Ohlstein, Julio C. Sanmiguel, A. Tretiakova, Soumitra Roy, S. Worgall, James M. Wilson, M. Limberis
Human gene therapy, 2021

Semantic Scholar DOI PubMed
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APA
Tycko, J., Adam, V., Crosariol, M., Ohlstein, J., Sanmiguel, J. C., Tretiakova, A., … Limberis, M. (2021). Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways. Human Gene Therapy.

Chicago/Turabian
Tycko, Josh, V. Adam, M. Crosariol, J. Ohlstein, Julio C. Sanmiguel, A. Tretiakova, Soumitra Roy, S. Worgall, James M. Wilson, and M. Limberis. “Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.” Human gene therapy (2021).

MLA
Tycko, Josh, et al. “Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.” Human Gene Therapy, 2021.


Abstract

Infants and older adults are especially vulnerable to infection by respiratory syncytial virus (RSV), which can cause significant illness and irreparable damage to the lower respiratory tract and for which an effective vaccine is not readily available. Palivizumab, a recombinant monoclonal antibody (mAb), is an approved therapeutic for RSV infection for use in high-risk infants only. Due to several logistical issues, including cost of goods and scale-up limitations, palivizumab is not approved for other populations that are vulnerable to severe RSV infections, such as older adults. In this study, we demonstrate that intranasal delivery of adeno-associated virus serotype 9 (AAV9) vector expressing palivizumab or motavizumab, a second-generation version of palivizumab, significantly reduced the viral load in the lungs of the BALB/c mouse model of RSV infection. Notably, we demonstrate that AAV9 vector-mediated prophylaxis against RSV was effective despite the presence of serum-circulating neutralizing AAV9 antibodies. These findings substantiate the feasibility of repeatedly administering AAV9 vector to the airway for seasonal prophylaxis against RSV, thereby expanding the application of vectored delivery of mAbs as an effective prophylaxis strategy against various airborne viruses.


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