I love taking systematic experimental approaches that enable progress towards a quantitative understanding of gene expression while keeping the door open for unexpected biological discoveries. Recently, we developed a new approach, HT-recruit, to measure how domains from all proteins in the human nucleus can affect gene expression when recruited to a target gene. We discovered transcriptional effector domains in 600 human proteins and shed new light on the evolution of major transcription factor families, like KRAB and Hox.
My long-term goal is to apply this knowledge to develop safe gene therapies that cure diseases. I have employed a variety of high-throughput methods to better measure, model, and mitigate CRISPR gene editing off-target activity, working in both academia and at a biotech startup.
I am currently working on my Ph.D. in the labs of Michael Bassik and Lacra Bintu with support from the NIH F99/K00 Fellowship. At Stanford, I am a teaching assistant for a course on the science and bioethics of CRISPR, was an instructor for a hands-on cell engineering lab, and mentor our amazing iGEM team. As a BioFutures Fellow and co-leader of The Genetics Advocacy Committee, I collaborated with fellow trainees to critically analyze and improve graduate STEM training.
Please reach out if you would like to chat!
Josh Tycko, Virginie S Adam, Marco Crosariol, Jason Ohlstein, Julio Sanmiguel, Anna P Tretiakova, Soumitra Roy, Stefan Worgall, James M Wilson, Maria P Limberis
Human Gene Therapy, 2021 Jul 19
Marinov Gk, Kim Sh, Bagdatli St, Trevino Ae, Tycko J, Wu T, Bintu L, Bassik Mc, He C, He C, Kundaje A, Greenleaf Wj
bioRxiv, 2021 Mar 16
Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, Abhimanyu Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn Spees, Peter Suzuki, Georgi K. Marinov, Anshul Kundaje, Michael C. Bassik, Lacramioara Bintu
Cell, vol. 183(7), 2020 Nov 22
Theresa Maier, Nicolas James Wheeler, Nicolas James Wheeler, Erica K. O. Namigai, Josh Tycko, Richard Ernest Grewelle, Yimtubezinash Woldeamanuel, Katharina Klohe, Javier Perez-Saez, Susanne H. Sokolow, Susanne H. Sokolow, Giulio A. De Leo, Timothy P. Yoshino, Mostafa Zamanian, Jutta Reinhard-Rupp
PLOS Neglected Tropical Diseases, vol. 13(12), 2019 Nov 18
Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik
Nature Communications, vol. 10(1), 2019 Aug 5