About

I develop new high-throughput, synthetic biology technologies to manipulate and measure human gene regulation.

I love taking systematic experimental approaches that enable progress towards a quantitative understanding of gene expression while keeping the door open for unexpected biological discoveries. Recently, we developed a new approach, HT-recruit, to measure how domains from all proteins in the human nucleus can affect gene expression when recruited to a target gene. We discovered transcriptional effector domains in 600 human proteins and shed new light on the evolution of major transcription factor families, like KRAB and Hox.

My long-term goal is to apply this knowledge to develop safe gene therapies that cure diseases. I have employed a variety of high-throughput methods to better measure, model, and mitigate CRISPR gene editing off-target activity, working in both academia and at a biotech startup.

I am currently working on my Ph.D. in the labs of Michael Bassik and Lacra Bintu with support from the NIH F99/K00 Fellowship. At Stanford, I am a teaching assistant for a course on the science and bioethics of CRISPR, was an instructor for a hands-on cell engineering lab, and mentor our amazing iGEM team. As a BioFutures Fellow and co-leader of The Genetics Advocacy Committee, I collaborated with fellow trainees to critically analyze and improve graduate STEM training.

Please reach out if you would like to chat!

Publications

Adeno-Associated Virus Vector-Mediated Expression of Antirespiratory Syncytial Virus Antibody Prevents Infection in Mouse Airways.

Josh Tycko, Virginie S Adam, Marco Crosariol, Jason Ohlstein, Julio Sanmiguel, Anna P Tretiakova, Soumitra Roy, Stefan Worgall, James M Wilson, Maria P Limberis

Human Gene Therapy, 2021 Jul 19

Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)

Marinov Gk, Kim Sh, Bagdatli St, Trevino Ae, Tycko J, Wu T, Bintu L, Bassik Mc, He C, He C, Kundaje A, Greenleaf Wj

bioRxiv, 2021 Mar 16

High-Throughput Discovery and Characterization of Human Transcriptional Effectors.

Josh Tycko, Nicole DelRosso, Gaelen T. Hess, Aradhana, Abhimanyu Banerjee, Aditya Mukund, Mike V. Van, Braeden K. Ego, David Yao, Kaitlyn Spees, Peter Suzuki, Georgi K. Marinov, Anshul Kundaje, Michael C. Bassik, Lacramioara Bintu

Cell, vol. 183(7), 2020 Nov 22

Gene drives for schistosomiasis transmission control

Theresa Maier, Nicolas James Wheeler, Nicolas James Wheeler, Erica K. O. Namigai, Josh Tycko, Richard Ernest Grewelle, Yimtubezinash Woldeamanuel, Katharina Klohe, Javier Perez-Saez, Susanne H. Sokolow, Susanne H. Sokolow, Giulio A. De Leo, Timothy P. Yoshino, Mostafa Zamanian, Jutta Reinhard-Rupp

PLOS Neglected Tropical Diseases, vol. 13(12), 2019 Nov 18

Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik

Nature Communications, vol. 10(1), 2019 Aug 5

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