Josh Tycko

Systematic discovery of protein functions in human cells to understand gene regulation and enable genetic medicine

Genetic and epigenetic editing


Publications


Multicenter integrated analysis of noncoding CRISPRi screens


David Yao*, Josh Tycko*#, Jin Woo Oh*, Lexi R. Bounds*, Sager J. Gosai*, Lazaros Lataniotis*, Ava Mackay-Smith, Benjamin R. Doughty, I. Gabdank, Henri Schmidt, Tania Guerrero-Altamirano, Keith Siklenka, Katherine Guo, Alexander D White, Ingrid Youngworth, Kalina Andreeva, Xingjie Ren, Alejandro Barrera, Yunhai Luo, G. Yardımcı, R. Tewhey, A. Kundaje, William J. Greenleaf, P. Sabeti, Christina S. Leslie, Y. Pritykin, Jill E. Moore, Michael A Beer, C. Gersbach, Timothy E. Reddy, Yin Shen, Jesse M Engreitz, M. Bassik, Steven K. Reilly#

Nature Methods, 2024


CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping


G. Marinov, Samuel H. Kim, S. Bagdatli, Soon Il Higashino, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, M. Bassik, Chuan He, A. Kundaje, W.J. Greenleaf

Genome Biology, 2023


Development of compact transcriptional effectors using high-throughput measurements in diverse contexts


Josh Tycko, Mike V. Van, Aradhana, Nicole DelRosso, David Yao, Xiaoshu Xu, Connor Ludwig, Kaitlyn K. Spees, Kathy Liu, Gaelen T. Hess, Mingxin Gu, Aditya Mukund, Peter H. Suzuki, Roarke A. Kamber, Lei S. Qi, Lacramioara Bintu, M. Bassik

bioRxiv, 2023


A genome-wide genetic screen uncovers determinants of human pigmentation


V. Bajpai, T. Swigut, Jaaved Mohammed, S. Naqvi, M. Arreola, Josh Tycko, Tayne C. Kim, J. Pritchard, M. Bassik, Joanna Wysocka

Science, 2023


Modeling the efficacy of CRISPR gene drive for snail immunity on schistosomiasis control


R. Grewelle, J. Perez-Saez, Josh Tycko, E. Namigai, Chloe Rickards, G. D. De Leo

PLoS Neglected Tropical Diseases, 2022


Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements


Josh Tycko*, M. Wainberg*, G. Marinov*, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa N Truong, Alexandro E. Trevino, Kaitlyn K. Spees, David Yao, Irene M. Kaplow, P. Greenside, D. Morgens, D. Phanstiel, M. Snyder, Lacramioara Bintu, W. Greenleaf, A. Kundaje, M. Bassik

Nature Communications, 2019


Identification of Guide-Intrinsic Determinants of Cas9 Specificity


Nicholas C. Huston, Josh Tycko, Eric L Tillotson, Christopher J. Wilson, V. Myer, H. Jayaram, Barrett E Steinberg

The CRISPR Journal, 2019


Gene drives for schistosomiasis transmission control


T. Maier, Nicolas J. Wheeler, E. Namigai, Josh Tycko, R. Grewelle, Y. Woldeamanuel, Katharina Klohe, J. Perez-Saez, S. Sokolow, G. D. De Leo, T. Yoshino, M. Zamanian, J. Reinhard-Rupp

PLoS Neglected Tropical Diseases, 2019


Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells


Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, J. Gootenberg, O. Abudayyeh, V. Myer, Christopher J. Wilson, P. Hsu

Nature Communications, 2018


Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.


Gaelen T. Hess*, Josh Tycko*, David Yao*, M. Bassik

Molecular Cell, 2017


Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.


Josh Tycko, Vic Myer, Patrick Hsu

Molecular Cell, 2016




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