Journal article
Connor H. Ludwig, A. Thurm, D. Morgens, Kevin J. Yang, Josh Tycko, M. Bassik, B. Glaunsinger, Lacramioara Bintu
bioRxiv, 2022
bioRxiv, 2022
Semantic Scholar
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APA
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Ludwig, C. H., Thurm, A., Morgens, D., Yang, K. J., Tycko, J., Bassik, M., … Bintu, L. (2022). High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells. BioRxiv.
Chicago/Turabian
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Ludwig, Connor H., A. Thurm, D. Morgens, Kevin J. Yang, Josh Tycko, M. Bassik, B. Glaunsinger, and Lacramioara Bintu. “High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells.” bioRxiv (2022).
MLA
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Ludwig, Connor H., et al. “High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells.” BioRxiv, 2022.
BibTeX Click to copy
@article{connor2022a,
title = {High-Throughput Discovery and Characterization of Viral Transcriptional Effectors in Human Cells},
year = {2022},
journal = {bioRxiv},
author = {Ludwig, Connor H. and Thurm, A. and Morgens, D. and Yang, Kevin J. and Tycko, Josh and Bassik, M. and Glaunsinger, B. and Bintu, Lacramioara}
}
Abstract
Viruses encode transcriptional regulatory proteins critical for controlling viral and host gene expression. Given their multifunctional nature and high sequence divergence, it is unclear which viral proteins can affect transcription and which specific sequences contribute to this function. Using a high-throughput assay, we measured the transcriptional regulatory potential of over 60,000 protein tiles across ∼1,500 proteins from 11 coronaviruses and all nine human herpesviruses. We discovered hundreds of new transcriptional effector domains, including a conserved repression domain in all coronavirus Spike homologs, dual activation-repression domains in VIRFs, and an activation domain in six herpesvirus homologs of the single-stranded DNA-binding protein that we show is important for viral replication and late gene expression in KSHV. For the effector domains we identified, we investigated their mechanisms via high-throughput sequence and chemical perturbations, pinpointing sequence motifs essential for function. This work massively expands viral protein annotations, serving as a springboard for studying their biological and health implications and providing new candidates for compact gene regulation tools.
