Josh Tycko

Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



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Josh Tycko

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Curriculum vitae



Department of Neurobiology

Harvard Medical School




Josh Tycko

Systematic discovery of protein functions in human cells to understand gene regulation and enable gene therapy



Department of Neurobiology

Harvard Medical School



About


I develop high-throughput synthetic biology technologies to manipulate and measure human gene regulation.

Recently, we developed a new approach, HT-recruit, to measure how domains from all proteins in the human nucleus can affect gene expression when recruited to a target gene. We discovered transcriptional effector domains in 600 human proteins and shed new light on the evolution of major transcription factor families, like KRAB and Hox, while also engineering improved activator and repressor domains for synthetic biology.

My long-term goal is to apply our discoveries to develop safe genetic medicines that cure diseases. I have discovered efficient recombinases for large DNA integration in human cells and employed a variety of high-throughput methods to better measure, model, and mitigate CRISPR gene editing off-target activity, working in both academia and at a biotech startup.

I completed my Ph.D. in the labs of Michael Bassik and Lacra Bintu with support from the NIH F99/K00 Fellowship.  At Stanford, I was a teaching assistant for a course on the science and bioethics of CRISPR, was an instructor for a hands-on cell engineering lab, and mentor to our amazing iGEM team. As a BioFutures Fellow and co-leader of The Genetics Advocacy Committee, I collaborated with fellow trainees to critically analyze and improve graduate STEM training.

Please reach out if you would like to chat!
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